기계공학부 구성원들의 많은 관심과 참여 부탁드립니다.

▣ 주   제: 3D-printed transparent and high-density electrocorticography array for simultaneous cortex-wide electrophysiology and optical imaging

▣ 연   사: 이지용 박사

▣ 소   속: University of Minnesota

▣ 일   시: 2024. 05. 01.(수) 16:00

▣ 장   소: 제1공학관 A442호

▣ 초   청: 류원형 교수

▣ 초   록

High-density, micrometer-scale electrocorticography (ECoG) is a widely used neural recording method for measuring brain activity with high temporal resolution across distributed sites on the brain surface. Meanwhile, optical calcium imaging offers deep and detailed views of neural activity at the cellular level across the cortex. A technology that enables simultaneous calcium imaging and ECoG recordings would combine the advantages of both methods, facilitating comprehensive neural activity measurements across diverse brain regions through multi-modal approaches. We've developed a 64-channel high-density ECoG array using a customized 3D printer, allowing simultaneous ECoG and calcium imaging across the dorsal cortex in mice. Before printing, we employed a laser scanning system to ensure printing accuracy. The printed electrodes, with a width of 60 μm, exhibited promising electrical properties for signal recording. Additionally, the 3D-printed PEDOT:PSS traces with insulation layer showed high transmittance in the visible light range, enabling calcium imaging in live mice. Implanting these arrays in animals caused no delamination or adverse effects over an eight-week period. Our next steps involve using 1P or 2P microscopes to image brain activity and study the correlation between calcium activities and local field potentials. This technology represents a significant advancement, promising detailed, long-term studies of brain activity at the single-cell level.