기계공학부 구성원들의 많은 관심과 참여 부탁드립니다.

▣ 제  목: 미세유체공학기반 인공엑소좀 플랫폼: 바이오의약품 산업의 적용 가능성
▣ 연  사: 박선영

▣ 소  속: 강원대학교 의생명융합학부

▣ 일  시: 2025. 10. 17.(Fri) 13:00 / 16:00

▣ 장  소: 제2공학관 B040호

▣ 초  록

Exosomes are nanoscale lipid-bilayer vesicles that mediate intercellular communication by transporting bioactive molecules such as nucleic acids and proteins, and they have emerged as promising candidates for regenerative medicine. Among them, salivary gland epithelial stem cell-derived exosomes (sgESC-Exos) exert antifibrotic effects in salivary gland injury models through the delivery of microRNAs, including miR-1290 and miR-3162. However, their clinical application remains limited by low production yield and particle heterogeneity. To overcome these barriers, we developed exosome-mimetic nanoparticles (ENPs) using a microfluidic platform integrated with a reverse-Tesla micromixer. This platform enables efficient mixing and self-assembly of lipid and aqueous phases, generating uniform nanoparticles with high nucleic acid encapsulation efficiency. The resulting ENPs exhibit physicochemical properties comparable to natural exosomes while displaying improved homogeneity. Functionally, ENPs demonstrated markedly enhanced therapeutic efficacy, with approximately 21-fold higher cellular uptake and a two-fold faster wound closure rate compared to sgESC-Exos. These findings highlight the potential of microfluidics-based bottom-up synthesis as a robust strategy for engineering exosome-inspired therapeutics, paving the way for clinical translation in regenerative medicine.