[BK세미나] 4/16(목) 김문일 교수(가천대학교) "Active site engineering of nanozymes for advanced point-of-care biosensing and beyond"

기계공학부 구성원들의 많은 관심과 참여 부탁드립니다.

▣ 주 제: Active site engineering of nanozymes for advanced point-of-care biosensing and beyond

▣ 연 사: 김문일 교수

▣ 소 속: 가천대학교 바이오나노학과

▣ 일 시: 2026. 4. 16.(목) 17:00

▣ 장 소: 제4공학관 D603호

▣ 초 록

Nanozymes have emerged as robust and cost-effective alternatives to natural enzymes, offering high stability and tunable catalytic properties. In this presentation, I will discuss recent advances in active-site engineering of nanozymes, focusing on the regulation of catalytic activity and reaction selectivity for advanced point-of-care (POC) biosensing applications. First, I will introduce cobalt-doped mesoporous cerium oxide (Co-m-ceria), which exhibits exceptionally high peroxidase-like activity while suppressing oxidase-like activity under near-neutral pH, achieving nearly 600-fold higher catalytic efficiency than pristine ceria through dopant engineering guided by density functional theory. This platform further enables multiplexed biomarker detection via enzyme immobilization within mesoporous structures and integration into paper-based microfluidic devices. Next, I will present nanoflower-type hybrid nanozymes, including DNA–copper, manganese–copper, and cysteine–histidine–copper systems, which exhibit laccase-like activity and enable efficient colorimetric detection of phenolic targets. I will then highlight our recent work on single-atom nanozymes, particularly Cu–N/O coordinated aerogel nanozymes with dual enzymatic activities, enabling simultaneous detection of multiple neurotransmitters in POC platforms, as well as emerging strategies such as out-of-plane ligand coordination in Ru-based single-atom nanozymes, which allows selective catalytic pathways by suppressing competing reactions under near-neutral conditions. Finally, I will present paper-based microfluidic systems incorporating these nanozymes for rapid and visual detection in resource-limited environments, demonstrating that precise control of active sites and catalytic microenvironments is a key strategy for advancing nanozyme-based biosensing and expanding their applications beyond diagnostics.